Publications

2021

  • C-di-AMP is essential for the virulence of Enterococcus faecalis. Kundra S., Lam L.N, Kajfasz J.K., Casella L. G., Andersen M.J., Abranches J., Flores-Mireles A.L. and Lemos JA. Infect Immu. 2021 Aug. [Read more…]
  • Amyloid aggregation of Streptococcus mutans Cnm influences its collagen-binding activity. Cologna. N.M, Samaddar S., Valle C.A, Vargas J., Aviles-Reyes A., Morales J., Ganguly T., Pileggi R., Brady L. J., Lemos J.A and Abranches J. App Enviro Micro. 2021 Aug. [Read more…]
  • Detection of Streptococcus mutans in symptomatic and asymptomatic infected root canals. Lima A. R, Herrera D.R, Francisco P.A, Pereira A. C, Lemos J.A, Abranches J. and Gomes B. Clin Oral Investig. 2021 Jun. [Read more…]
  • Association of Candida albicans and Cbp + Streptococcus mutans with early childhood caries recurrence infections. Garcia BA, Acosta NC, Tomar SL, Roesch LFW, Lemos JA, Mugayar LCF, Abranches J. Sci Rep. May 2021. DOI: 10.1038/s41598-021-90198-3 [Read more…]
  • Zinc Import Mediated by AdcABC is Critical for Colonization of the Dental Biofilm by Streptococcus mutans in an Animal Model.. Ganguly T, Peterson AM, Kajfasz JK, Abranches J, Lemos JA. Mol Oral Microbiol. 2021 Jun. [Read more…]
  • A Modular Synthetic Route Involving N-Aryl-2-Nitrosoaniline Intermediates Leads to a New Series of 3-Substituted Halogenated Phenazine Antibacterial Agents. Yang H, Kundra S, Chojnacki M, Liu K, Fuse M, Abouelhassan Y, Kallifidas D, Zhang P, Haung G, Jin S, Ding Y, Luesch H, Rohde K, Dunman P, Lemos JA, Huigens III R. J. Med. Chem. 2021 Apr. [Read more…]
  • Increased oxidative stress tolerance of a spontaneously-occurring perR gene mutation in Streptococcus mutans UA159. Kajfasz JK, Zuber P, Ganguly T, Abranches J, Lemos JA. J. Bacteriol. 2021 Mar. [Read more…]

2020

  • Survival of the fittest: the relationship of (p)ppGpp with bacterial virulence. Kundra S, Colomer-Winter C, Lemos JA. Front Microbiol. 2020 Dec. DOI:10.3389/fmicb.2020.601417. [Read more…]
  • Detection of Streptococcus mutans in symptomatic and asymptomatic infected root canals. Lima AR, Herrera DR, Francisco PA, Pereira AC, Lemos JA, Abranches J, Gomes BPFA. Clin Oral Investig. 2020 Nov. DOI:10.1007/s00784-020-03676-9. [Read more…]
  • Phenotypic and genotypic characterization of Streptococcus mutans strains isolated from endodontic infections. Augusto R.Lima, Tridib Ganguly, Alejandro R.Walker, Natalia Acosta, Priscila A.Francisco, Roberta Pileggi, José A.Lemos, Brenda P.F. A.Gomes, Jacqueline Abranches. J. Endodontics. 2020 Sept 11. DOI:10.1016/j.joen.2020.09.002. [Read more…]
  • Regulatory circuits controlling Spx levels in Streptococcus mutans. Tridib Ganguly  Jessica K. Kajfasz  Jacqueline Abranches  José A. Lemos. Mol Micro. 2020 March 19. [Read more…]
  • Manganese uptake, mediated by SloABC and MntH, is essential for the fitness of Streptococcus mutans. Kajfasz JK, Katrak C, Ganguly T, Vargas J, Wright L, Peters ZT, Spatafora GA, Abranches J, Lemos JA. mSphere. 2020 Jan 8;5(1). pii: e00764-19. doi: 10.1128/mSphere.00764-19. [Read more…]
  • PepO is a target of the two-component systems VicRK and CovR required for systemic virulence of Streptococcus mutans. Lívia A. Alves, Tridib Ganguly, Érika N. Harth-Chú, Jessica Kajfasz, José A. Lemos, Jacqueline Abranches, and Renata O. Mattos-Granera. Virulence. 2020; 11(1): 521–536. DOI: 10.1080/21505594.2020.1767377. [Read more…]

2019

  • (p)ppGpp and CodY promote Enterococcus faecalis virulence in a murine model of catheter-associated urinary tract infection. Colomer-Winter C, Flores-Mireles AL, Kundra S, Hultgren SJ, Lemos JA. mSphere. 2019 Jul 24;4(4). pii: e00392-19. doi: 10.1128/mSphere.00392-19. [Read more…]
  • Adaptation to adversity: the intermingling of stress tolerance and pathogenesis in Enterococci. Gaca AO, Lemos JA. Microbiol Mol Biol Rev. 2019 Jul 17;83(3). pii: e00008-19. doi: 10.1128/MMBR.00008-19. Print 2019 Aug 21. [Read more…]
  • Biofilm assays on fibrinogen-coated silicone catheters and 96-well polystyrene plates. Colomer-Winter C, Lemos JA, Fores-Mireles AL. Bio Protoc. 2019 Mar 20;9(6). pii: e3196. doi: 10.21769/BioProtoc.3196. [Read more…]
  • The biology of Streptococcus mutans. Lemos Ja, Palmer SR, Zeng L, Wen ZT, Kajfasz JK, Freires IA, Abranches J, Brady LJ. Microbiol Spectr. 2019 Jan;7(1). doi: 10.1128/microbiolspec.GPP3-0051-2018. [Read more…]

2018

  • Basal levels of (p)ppGpp differentially affect the pathogenesis of infective endocarditis in Enterococcus faecalis. Colomer-Winter C, Gaca AP, Chuang-Smith ON, Lemos JA, Frank KL. Microbiology. 2018 Oct;164(10):1254-1265. doi: 10.1099/mic.0.000703. [Read more…]
  • Biology of oral Streptococci. Abranches J, Zeng L, Kajfasz JK, Palmer SR, Chakraborty B, Wen ZT, Richards VP, Brady LJ, Lemos JA. Microbiol Spectr. 2018 Oct;6(5). doi: 10.1128/microbiolspec.GPP3-0042-2018. [Read more…]
  • Managanese acquisition is essential for virulence of Enterococcus faecalis. Colomer-Winter C, Flores-Mireles AL, Baker SP, Frank KL, Lynch AJL, Hultgren SJ, Kitten T, Lemos JA. PloS Pathog. 2018 Sep 20;14(9):e1007102. doi: 10.1371/journal.ppat.1007102. [Read more…]
  • Disruption of a novel iron transport system reverses oxidative stress phenotypes of a dpr mutant strain of Streptococcus mutans. Ganguly T, Kajfasz JK, Miller JH, Rabinowitz E, Galvao LCC, Rosalen PL, Abranches J, Lemos JA. J Bacteriol. 2018 Jun 25;200(14). pii: e00062-18. doi: 10.1128/JB.00062-18. [Read more…]
  • Whole genome sequence and phenotypic characterization of a Cbm+ serotype e strain of Streptococcus mutans. Aviles-Reyes A, Freires IA, Kajfasz JK, Barbieri D, Miller JH, Lemos JA, Abranches J. Mol Oral Microbiol. 2018 Jun;33(3):257-269. doi: 10.1111/omi.12222. [Read more…]
  • Characterization of the pgf operon involved in the post-translational modification of Streptococcus mutans surface proteins. Aviles-Reyes A, Freires IA, Besingi R, Purushotham S, Deivanayagam C, Brady LJ, Abranches J, Lemos JA. Sci Rep. 2018 Mar 16;8(1):4705. doi: 10.1038/s41598-018-23170-3. [Read more…]

2017

  • A new perspective of an old villain: Revisiting biomarkers of caries development. Freires IA, Lemos JA, Abranches J. EbioMedicine. 2017 Nov;25:14-15. doi: 10.1016/j.ebiom.2017.10.022. [Read more…]
  • Transcriptome responses of Streptococcus mutans to peroxide stress: identification of novel antioxidant pathways regulated by Spx. Kajfasz JK, Ganguly T, Hardin EL, Abranches J, Lemos JA. Sci Rep. 2017 Nov 22;7(1):16018. doi: 10.1038/s41598-017-16367-5. [Read more…]
  • Association of metal homeostasis and (p)ppGpp regulation in the pathophysiology of Enterococcus faecalis. Colomer-Winter C, Gaca AP, Lemos JA. Infect Immun. 2017 Jun 20;85(7). pii: e00260-17. doi: 10.1128/IAI.00260-17. [Read more…]

2016

  • Simultaneous spatiotemporal mapping of in situ pH and bacterial activity within an intact 3D microcolony structure. Hwang G, Liu Y, Kim D, Sun V, Aviles-Reyes A, Kajfasz JK, Lemos JA, Koo H. Sci Rep. 2016 Sep 8;6:32841. doi: 10.1038/srep32841. [Read more…]
  • Stress Physiology of Lactic Acid Bacteria. Papadimitriou K, Alegría Á, Bron PA, de Angelis M, Gobbetti M, Kleerebezem M, Lemos JA, Linares DM, Ross P, Stanton C, Turroni F, van Sinderen D, Varmanen P, Ventura M, Zúñiga M, Tsakalidou E, Kok J. Microbiol Mol Biol Rev. 2016 Jul 27;80(3):837-90. doi: 10.1128/MMBR.00076-15. Review. PMID: 27466284. [Read more…]
  • The CtsR regulator controls the expression of clpC, clpE and clpP and is required for the virulence of Enterococcus faecalis in an invertebrate model. Cassenego AP, de Oliveira NE, Laport MS, Abranches J, Lemos JA, Giambiagi-deMarval M. Antonie Van Leeuwenhoek. 2016 Sep;109(9):1253-9. doi: 10.1007/s10482-016-0727-0. PMID: 27388279. [Read more…]
  • Heterologous expression of Streptococcus mutans Cnm in Lactococcus lactis promotes intracellular invasion, adhesion to human cardiac tissues and virulence. Freires IA, Avilés-Reyes A, Kitten T, Simpson-Haidaris PJ, Swartz M, Knight PA, Rosalen PL, Lemos JA, Abranches J. Virulence. 2016 Jun 3:1-12. PMID: 27260618. [Read more…]
  • Inactivation of the spxA1 or spxA2 gene of Streptococcus mutans decreases virulence in the rat caries model. Galvão LC, Rosalen PL, Rivera-Ramos I, Franco GC, Kajfasz JK, Abranches J, Bueno-Silva B, Koo H, Lemos JA. Mol Oral Microbiol. 2016 Apr 1. doi: 10.1111/omi.12160. PMID: 27037617. [Read more…]
  • The Collagen Binding Proteins of Streptococcus mutans and Related Streptococci. Avilés-Reyes A, Miller JH, Lemos JA, Abranches J. Mol Oral Microbiol. 2016 Mar 15. doi: 10.1111/omi.12158. Review. PMID: 26991416. [Read more…]

2015

  • From (p)ppGpp to (pp)pGpp: Characterization of Regulatory Effects of pGpp Synthesized by the Small Alarmone Synthetase of Enterococcus faecalis. Gaca AO, Kudrin P, Colomer-Winter C, Beljantseva J, Liu K, et al. Journal of bacteriology.2015; 197(18):2908-19. [Read more…]
  • Transcription of Oxidative Stress Genes Is Directly Activated by SpxA1 and, to a Lesser Extent, by SpxA2 in Streptococcus mutans. Kajfasz JK, Rivera-Ramos I, Scott-Anne K, Gregoire S, Abranches J, et al. Journal of bacteriology. 2015; 197(13):2160-70. [Read more…]
  • Transcriptional profile of glucose-shocked and acid-adapted strains of Streptococcus mutans. Baker JL, Abranches J, Faustoferri RC, Hubbard CJ, Lemos JA, et al.Molecular oral microbiology. 2015. [Read more…]
  • The collagen binding protein Cnm contributes to oral colonization and cariogenicity of Streptococcus mutans OMZ175. Miller JH, Avilés-Reyes A, Scott-Anne K, Gregoire S, Watson GE, et al. Infection and immunity. 2015; 83(5):2001-10. [Read more…]
  • Many means to a common end: the intricacies of (p)ppGpp metabolism and its control of bacterial homeostasis. Gaca AO, Colomer-Winter C, Lemos JA. Journal of bacteriology. 2015; 197(7):1146-56. [Read more…]
  • Transcriptional and Phenotypic Characterization of Novel Spx-Regulated Genes in Streptococcus mutans. Galvão LC, Miller JH, Kajfasz JK, Scott-Anne K, Freires IA, et al. PloS one. 2015; 10(4):e0124969. [Read more…]

2014

  • Modification of Streptococcus mutans Cnm by PgfS contributes to adhesion, endothelial cell invasion, and virulence. Avilés-Reyes A, Miller JH, Simpson-Haidaris PJ, Hagen FK, Abranches J, et al.Journal of bacteriology. 2014; 196(15):2789-97. [Read more…]
  • Streptococcus mutans NADH oxidase lies at the intersection of overlapping regulons controlled by oxygen and NAD+ levels. Baker JL, Derr AM, Karuppaiah K, MacGilvray ME, Kajfasz JK, et al. Journal of bacteriology. 2014; 196(12):2166-77. [Read more…]
  • Cnm is a major virulence factor of invasive Streptococcus mutans and part of a conserved three-gene locus. Avilés-Reyes A, Miller JH, Simpson-Haidaris PJ, Lemos JA, Abranches J. Molecular oral microbiology. 2014; 29(1):11-23. [Read more…]
  • The cell wall-targeting antibiotic stimulon of Enterococcus faecalis. Abranches J, Tijerina P, Avilés-Reyes A, Gaca AO, Kajfasz JK, et al. PloS one. 2014; 8(6):e64875. [Read more…]
  • Transcriptome analysis of Enterococcus faecalis during mammalian infection shows cells undergo adaptation and exist in a stringent response state. Frank KL, Colomer-Winter C, Grindle SM, Lemos JA, Schlievert PM, et al. PloS one. 2014; 9(12):e115839. [Read more…]

2013

  • Basal levels of (p)ppGpp in Enterococcus faecalis: the magic beyond the stringent response. Gaca AO, Kajfasz JK, Miller JH, Liu K, Wang JD, et al. mBio. 2013; 4(5):e00646-13. [Read more…]
  • Streptococcus mutans: a new Gram-positive paradigm? Lemos JA, Quivey RG Jr, Koo H, Abranches J. Microbiology (Reading, England). 2013; 159(Pt 3):436-45. [Read more…]
  • Phenotypic heterogeneity of genomically-diverse isolates of Streptococcus mutans. Palmer SR, Miller JH, Abranches J, Zeng L, Lefebure T, et al. PloS one.2013; 8(4):e61358. [Read more…]

2012

  • Novel antibiofilm chemotherapy targets exopolysaccharide synthesis and stress tolerance in Streptococcus mutans to modulate virulence expression in vivo. Falsetta ML, Klein MI, Lemos JA, Silva BB, Agidi S, et al. Antimicrobial agents and chemotherapy. 2012; 56(12):6201-11. [Read more…]
  • Global transcriptional analysis of the stringent response in Enterococcus faecalis. Gaca AO, Abranches J, Kajfasz JK, Lemos JA. Microbiology (Reading, England).2012; 158(Pt 8):1994-2004. [Read more…]
  • The Spx regulator modulates stress responses and virulence in Enterococcus faecalis. Kajfasz JK, Mendoza JE, Gaca AO, Miller JH, Koselny KA, et al. Infection and immunity. 2012; 80(7):2265-75. [Read more…]
  • Role of (p)ppGpp in biofilm formation by Enterococcus faecalis. Chávez de Paz LE, Lemos JA, Wickström C, Sedgley CM. Applied and environmental microbiology. 2012; 78(5):1627-30. [Read more…]

2011

  • Transcriptome analysis reveals that ClpXP proteolysis controls key virulence properties of Streptococcus mutans. Kajfasz JK, Abranches J, Lemos JA. Microbiology (Reading, England). 2011; 157(Pt 10):2880-90. [Read more…]
  • The collagen-binding protein Cnm is required for Streptococcus mutans adherence to and intracellular invasion of human coronary artery endothelial cells. Abranches J, Miller JH, Martinez AR, Simpson-Haidaris PJ, Burne RA, et al. Infection and immunity. 2011; 79(6):2277-84. [Read more…]
  • ClpB, a class III heat-shock gene regulated by CtsR, is involved in thermotolerance and virulence of Enterococcus faecalis. de Oliveira NE, Abranches J, Gaca AO, Laport MS, Damaso CR, et al. Microbiology (Reading, England). 2011; 157(Pt 3):656-65. [Read more…]

2010

  • Dynamics of Streptococcus mutans transcriptome in response to starch and sucrose during biofilm development. Klein MI, DeBaz L, Agidi S, Lee H, Xie G, et al. PloS one. 2010; 5(10):e13478. [Read more…]
  • Characterization of the Streptococcus sobrinus acid-stress response by interspecies microarrays and proteomics. Martinez AR, Abranches J, Kajfasz JK, Lemos JA. Molecular oral microbiology. 2010; 25(5):331-42. [Read more…]
  • Two Spx proteins modulate stress tolerance, survival, and virulence in Streptococcus mutans. Kajfasz JK, Rivera-Ramos I, Abranches J, Martinez AR, Rosalen PL, et al. Journal of bacteriology. 2010; 192(10):2546-56. [Read more…]
  • Protocols to study the physiology of oral biofilms. Lemos JA, Abranches J, Koo H, Marquis RE, Burne RA. Methods in molecular biology (Clifton, N.J.). 2010; 666:87-102. [Read more…]

2009

  • Role of Clp proteins in expression of virulence properties of Streptococcus mutans. Kajfasz JK, Martinez AR, Rivera-Ramos I, Abranches J, Koo H, et al. Journal of bacteriology. 2009; 191(7):2060-8. [Read more…]
  • The molecular alarmone (p)ppGpp mediates stress responses, vancomycin tolerance, and virulence in Enterococcus faecalis. Abranches J, Martinez AR, Kajfasz JK, Chávez V, Garsin DA, et al. Journal of bacteriology. 2009; 191(7):2248-56. [Read more…]
  • Opportunities for disrupting cariogenic biofilms. Burne RA, Ahn SJ, Wen ZT, Zeng L, Lemos JA, et al. Advances in dental research. 2009; 21(1):17-20. [Read more…]

1998 – 2008

  • A model of efficiency: stress tolerance by Streptococcus mutans. Lemos JA, Burne RA. Microbiology (Reading, England). 2008; 154(Pt 11):3247-55. [Read more…]
  • Global regulation by (p)ppGpp and CodY in Streptococcus mutans. Lemos JA, Nascimento MM, Lin VK, Abranches J, Burne RA. Journal of bacteriology. 2008; 190(15):5291-9. [Read more…]
  • Role of RelA of Streptococcus mutans in global control of gene expression. Nascimento MM, Lemos JA, Abranches J, Lin VK, Burne RA. Journal of bacteriology. 2008; 190(1):28-36. [Read more…]
  • Three gene products govern (p)ppGpp production by Streptococcus mutans. Lemos JA, Lin VK, Nascimento MM, Abranches J, Burne RA. Molecular microbiology. 2007; 65(6):1568-81. [Read more…]
  • The oligopeptide (opp) gene cluster of Streptococcus mutans: identification, prevalence, and characterization. Nepomuceno RS, Tavares MB, Lemos JA, Griswold AR, Ribeiro JL, et al. Oral microbiology and immunology. 2007; 22(4):277-84. [Read more…]
  • Physiologic effects of forced down-regulation of dnaK and groEL expression in Streptococcus mutans. Lemos JA, Luzardo Y, Burne RA. Journal of bacteriology. 2007; 189(5):1582-8. [Read more…]
  • Organization of heat shock dnaK and groE operons of the nosocomial pathogen Enterococcus faecium. Laport MS, Dos Santos LL, Lemos JA, do Carmo F Bastos M, Burne RA, et al. Research in microbiology. 2006; 157(2):162-8. [Read more…]
  • Osmotic stress responses of Streptococcus mutans UA159. Abranches J, Lemos JA, Burne RA. FEMS microbiology letters. 2006; 255(2):240-6. [Read more…]
  • Characteristics of Streptococcus mutans strains lacking the MazEF and RelBE toxin-antitoxin modules. Lemos JA, Brown TA Jr, Abranches J, Burne RA. FEMS microbiology letters. 2005; 253(2):251-7. [Read more…]
  • A hypothetical protein of Streptococcus mutans is critical for biofilm formation. Brown TA Jr, Ahn SJ, Frank RN, Chen YY, Lemos JA, et al. Infection and immunity. 2005; 73(5):3147-51. [Read more…]
  • Role of HtrA in growth and competence of Streptococcus mutans UA159. Ahn SJ, Lemos JA, Burne RA. Journal of bacteriology. 2005; 187(9):3028-38. [Read more…]
  • Responses of cariogenic streptococci to environmental stresses. Lemos JA, Abranches J, Burne RA. Current issues in molecular biology. 2005; 7(1):95-107. [Read more…]
  • Adaptive acid tolerance response of Streptococcus sobrinus. Nascimento MM, Lemos JA, Abranches J, Gonçalves RB, Burne RA. Journal of bacteriology. 2004; 186(19):6383-90. [Read more…]
  • Transcriptional analysis of the groE and dnaK heat-shock operons of Enterococcus faecalis. Laport MS, Lemos JA, Bastos Md Mdo C, Burne RA, Giambiagi-De Marval M. Research in microbiology. 2004; 155(4):252-8. [Read more…]
  • Effects of RelA on key virulence properties of planktonic and biofilm populations of Streptococcus mutans. Lemos JA, Brown TA Jr, Burne RA. Infection and immunity. 2004; 72(3):1431-40. [Read more…]
  • Regulation and Physiological Significance of ClpC and ClpP in Streptococcus mutans. Lemos JA, Burne RA. Journal of bacteriology. 2002; 184(22):6357-66. [Read more…]
  • Genetic and physiologic analysis of the groE operon and role of the HrcA repressor in stress gene regulation and acid tolerance in Streptococcus mutans. Lemos JA, Chen YY, Burne RA. Journal of bacteriology. 2001; 183(20):6074-84. [Read more…]
  • Expression of the major heat shock proteins DnaK and GroEL in Streptococcus pyogenes: a comparison to Enterococcus faecalis and Staphylococcus aureus. Laport MS, de Castro AC, Villardo A, Lemos JA, Bastos MC, et al. Current microbiology. 2001; 42(4):264-8. [Read more…]
  • Molecular cloning, purification and immunological responses of recombinants GroEL and DnaK from Streptococcus pyogenes. Lemos JA, Burne RA, Castro AC. FEMS immunology and medical microbiology. 2000; 28(2):121-8. [Read more…]
  • Expression of heat-shock proteins in Streptococcus pyogenes and their immunoreactivity with sera from patients with streptococcal diseases. Lemos JA, Giambiagi-Demarval M, Castro AC. Journal of medical microbiology. 1998; 47(8):711-5. [Read more…]